K.C.S.E Biology Q & A - MODEL 2019PP2QN06
The table below shows the rate of product formation for two enzymes, H and J over a range of pH values
(a) On the same axis, plot graphs of the rate of product formation against pH.
(b) Account for the rate of product formation for enzyme H between:
(i) pH 1.0 and 3.0
(ii) pH 3.0 and 7.0.
(c) From the graph, determine: .
(i) the pH value at which the rate of product formation of the two enzymes was the same
(ii) the value of the rate of product formation for enzymes H and J at the pH value stated in (c)(i) above
(iii) the optimum pH value for enzyme J
(d) State one variable that may lead to the change in the optimum rate of product-formation of the two enzymes.
(e) Suggest with a reason, the likely part of the human alimentary canal where enzyme H would be found.
(b) (i) Rate of product formation increases with the increase in pH to the optimum then decreases;
pH 5 is the optimum pH value for activity of enzyme H; between pH 1 and 2 enzyme H/enzyme molecules are activated; Between pH 2 and 3 enzyme H (enzyme molecules) are denatured/destroyed; (. Enzyme is inactivated/ less active for denatured
(ii) Rate of product formation (sharply) decreases with increase in pH; Enzyme H is (not formed by increase in pH hence is denatured); No product forms above pH 4.0 to 7.0; because all enzymes (enzyme molecules) are denatured;
(c) (i) 4.0
(ii) 15.0 mg/hr
Acidic medium/Low pH